Lin S J, Chen J B, Hsu K T, Hwang D F
Department of Marine Food Science, National Taiwan Ocean University, Keelung, R.O.C.
J Nat Toxins. 1999 Feb;8(1):141-7.
Food poisoning due to ingestion of two fishes, Yongeichthys nebulosus and Sillago japonica, occurred in Kaohsiung, Taiwan, in February 1997. Two male persons (48 and 58 years old) were poisoned, with symptoms featured by dizziness, nausea, vomiting, numbness, and difficulty in respiration. All of the specimens of fish retained by the victims were combined and consisted of Yongeichthys nebulosus and Sillago japonica. These retained specimens were assayed for anatomical distribution of toxicity (as tetrodotoxin) and all specimens were found to be toxic. The highest toxicity of specimen was 7,650 mouse units (MU) in Y. nebulosus and 1,460 MU in S. japonica. However, the other specimens re-collected from that fish pier were also found to be highly toxic in Y. nebulosus, but nontoxic in S. japonica. Hence, Y. nebulosus was judged as the real causative fish in this food poisoning. The toxins were partially purified from the methanolic extracts of toxic fishes by ultrafiltration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis and high performance liquid chromatography analyses demonstrated that tetrodotoxin was the causative agent of this food poisoning.
1997年2月，台湾高雄发生因食用云斑海猪鱼和日本银鲈两种鱼类导致的食物中毒事件。两名男性（分别为48岁和58岁）中毒，症状表现为头晕、恶心、呕吐、麻木及呼吸困难。受害者留存的所有鱼样本合并后包括云斑海猪鱼和日本银鲈。对这些留存样本进行毒性（以河豚毒素计）的解剖分布测定，发现所有样本均有毒。云斑海猪鱼样本的最高毒性为7650小鼠单位（MU），日本银鲈样本的最高毒性为1460 MU。然而，从该鱼码头重新采集的其他样本中，云斑海猪鱼仍毒性很高，而日本银鲈无毒。因此，判定云斑海猪鱼是此次食物中毒的真正致病鱼类。通过超滤和生物凝胶P - 2柱色谱法从有毒鱼类的甲醇提取物中对毒素进行了部分纯化。醋酸纤维素膜电泳和高效液相色谱分析表明，河豚毒素是此次食物中毒的致病因子。
