Agrawal R, Deepika N U, Joseph R
Department of Food Microbiology, Central Food Technological Research Institute, Mysore, India.
Biotechnol Bioeng. 1999 Apr 20;63(2):249-52. doi: 10.1002/(sici)1097-0290(19990420)63:2<249::aid-bit14>3.0.co;2-d.
Variants of Aspergillus sp. and Penicillium sp. obtained after treatment with colchicine, ethyl methanesulphonate (EMS), or ultraviolet (UV) irradiation indicated varying levels of significant increases in their efficiency to transform alpha-pinene to verbenol. In case of Aspergillus sp. the UV-induced variant was the best performer with a 15-fold increase in biotransformation efficiency compared to the wild type. In case of colchicine and EMS-induced variants the biotransformation increases were 2- and 8-fold, respectively. The UV-induced variant of Penicillium sp. was capable of eight fold increase in efficiency while the colchicine- and EMS-induced variants were 1.5- and 2-fold, respectively. The variants were characterised with respect to changes in colony morphology, spore dimension, DNA content, and products formed, viz. verbenol and verbenone.
用秋水仙碱、甲基磺酸乙酯（EMS）或紫外线（UV）照射处理后获得的曲霉属和青霉属变种，在将α-蒎烯转化为马鞭草烯醇的效率上显示出不同程度的显著提高。对于曲霉属，紫外线诱导的变种表现最佳，与野生型相比，生物转化效率提高了15倍。对于秋水仙碱和EMS诱导的变种，生物转化分别提高了2倍和8倍。青霉属的紫外线诱导变种效率能够提高8倍，而秋水仙碱和EMS诱导的变种分别提高了1.5倍和2倍。对这些变种在菌落形态、孢子尺寸、DNA含量以及形成的产物（即马鞭草烯醇和马鞭草烯酮）方面的变化进行了表征。
