内吞膜回收需要钙内流。
Calcium influx is required for endocytotic membrane retrieval.
作者信息
Vogel S S, Smith R M, Baibakov B, Ikebuchi Y, Lambert N A
机构信息
Institute of Molecular Medicine and Genetics, Department of Medicine, Veterans Affairs Medical Center, Medical College of Georgia, 1120 15th Street CB 2803, Augusta, GA 30912-2630, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5019-24. doi: 10.1073/pnas.96.9.5019.
Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium-dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels-cadmium, omega-conotoxin MVIIC, omega-agatoxin IVA, and omega-agatoxin TK-blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through omega-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.
细胞利用内吞性膜回收来补偿胞吐作用后过量的表面膜。回收被认为是钙依赖性的，但这种钙的来源尚不清楚。我们发现，在海胆卵中，内吞性膜回收需要细胞外钙。P型钙通道抑制剂——镉、ω-芋螺毒素MVIIC、ω-阿加毒素IVA和ω-阿加毒素TK——会阻断膜回收；N型和L型通道的选择性抑制剂则不会。用钙离子载体处理以钙依赖性方式克服了阿加毒素的抑制作用。在受精后的前5分钟（即膜电位去极化的时期）施加镉会阻断膜回收。我们得出结论，通过ω-阿加毒素敏感通道的钙内流在信号传导以实现内吞性膜回收中起关键作用。
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