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Crystallization and preliminary X-ray studies of metallothionein II from rabbit liver.

作者信息

An Y, Li G, Ru B

机构信息

College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.

出版信息

Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1242-3. doi: 10.1107/s0907444999005430.

Abstract

Metallothionein II (Mw = 6.8 kDa), induced by cadmium and purified from rabbit liver, has been crystallized in space group P6222 or P6422. The unit-cell parameters were a = b = 113.4, c = 219.1 A, alpha = beta = 90.0, gamma = 120.0 degrees when crystallized from sodium citrate buffer and a = b = 113.4, c = 219.6 A, alpha = beta = 90.0, gamma = 120.0 degrees when crystallized from Tris-HCl buffer. There are 12 molecules per asymmetric unit and the solvent content is about 57%.

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