胆管疾病胆汁中幽门螺杆菌DNA的检测
Detection of Helicobacter DNA in bile from bile duct diseases.
作者信息
Roe I H, Kim J T, Lee H S, Lee J H
机构信息
Department of Internal Medicine, Dankook University College of Medicine, Chunan, Korea.
出版信息
J Korean Med Sci. 1999 Apr;14(2):182-6. doi: 10.3346/jkms.1999.14.2.182.
Several species of Helicobacter colonize the hepatobiliary tract of animals and cause hepatobiliary diseases. The aim of this study is to investigate Helicobacter found in the biliary tract diseases of humans. Thirty-two bile samples (15 from bile duct cancer, 6 from pancreatic head cancer, and 11 from intrahepatic duct stone) were obtained by percutaneous transhepatic biliary drainage. Polymerase chain reaction analysis using Helicobacter specific urease A gene and 16S rRNA primers, bile pH measurement, and Helicobacter culture were performed. Helicobacter DNA was detected in 37.5%, and 31.3% by PCR with ureA gene, and 16S rRNA, respectively. The bile pH was not related to the presence of Helicobacter. The cultures were not successful. In conclusion, Helicobacter can be detected in the bile of patients with bile duct diseases. The possibility of pathogenesis of biliary tract diseases in humans by these organisms will be further investigated.
几种幽门螺杆菌定殖于动物的肝胆管并引发肝胆疾病。本研究旨在调查人类胆道疾病中发现的幽门螺杆菌。通过经皮经肝胆道引流获取了32份胆汁样本（15份来自胆管癌，6份来自胰头癌，11份来自肝内胆管结石）。使用幽门螺杆菌特异性脲酶A基因和16S rRNA引物进行聚合酶链反应分析、测量胆汁pH值并进行幽门螺杆菌培养。分别用脲酶A基因和16S rRNA进行PCR检测时，幽门螺杆菌DNA的检出率分别为37.5%和31.3%。胆汁pH值与幽门螺杆菌的存在无关。培养未成功。总之，在胆管疾病患者的胆汁中可检测到幽门螺杆菌。这些微生物导致人类胆道疾病发病的可能性将进一步研究。
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