荷瘤小鼠脾脏中白细胞介素-1β信使核糖核酸表达增加与癌症恶病质有关吗？

Is increased IL-1 beta mRNA expression in spleen of tumor-bearing mice relevant to cancer cachexia?

作者信息

Nakatani S, Iwagaki H, Okabayashi T, Isozaki H, Takakura N, Tanaka N

机构信息

First Department of Surgery, Okayama University Medical School, Japan.

出版信息

Res Commun Mol Pathol Pharmacol. 1998 Dec;102(3):241-9.

PMID:10342911

Abstract

Using C3H/He mice bearing MH-134 tumor cells, cytokine inductions of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and interferon-gamma (IFN-gamma) were determined in spleens by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Levels of IL-1 beta were significantly (p<0.05) increased in the spleens of tumor-bearing mice compared with those of freely fed control animals. In contrast, no significant increase of other cytokine mRNAs was observed. In addition, constitutive levels of IL-1 beta mRNA were present in spleens of tumor-bearing mice during the experimental period. These results suggest that the over-expression of IL-1 beta mRNA was relevant to clinical manifestations of cancer-burden states.

摘要

利用携带MH - 134肿瘤细胞的C3H/He小鼠，通过逆转录聚合酶链反应（RT - PCR）分析测定脾脏中肿瘤坏死因子 - α（TNF - α）、白细胞介素 - 1β（IL - 1β）、白细胞介素 - 6（IL - 6）和干扰素 - γ（IFN - γ）的细胞因子诱导情况。与自由进食的对照动物相比，荷瘤小鼠脾脏中IL - 1β水平显著升高（p < 0.05）。相比之下，未观察到其他细胞因子mRNA的显著增加。此外，在实验期间，荷瘤小鼠脾脏中存在IL - 1β mRNA的组成型水平。这些结果表明，IL - 1β mRNA的过表达与癌症负荷状态的临床表现相关。