核小体重塑复合物Snf/Swi是体内转录维持所必需的，并且与组蛋白乙酰转移酶Gcn5部分冗余。
The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5.
作者信息
Sudarsanam P, Cao Y, Wu L, Laurent B C, Winston F
机构信息
Department of Genetics, Harvard Medical School, Boston, 200 Longwood Avenue, MA 02115, USA.
出版信息
EMBO J. 1999 Jun 1;18(11):3101-6. doi: 10.1093/emboj/18.11.3101.
Snf/Swi, a nucleosome remodeling complex, is important for overcoming nucleosome-mediated repression of transcription in Saccharomyces cerevisiae. We have addressed the mechanism by which Snf/Swi controls transcription in vivo of an Snf/Swi-dependent promoter, that of the SUC2 gene. By single-cell analysis, our results show that Snf/Swi is required for activated levels of SUC2 expression in every cell of a population. In addition, Snf/Swi is required for maintenance of SUC2 transcription, suggesting that continuous chromatin remodeling is necessary to maintain an active transcriptional state. Finally, Snf/Swi and Gcn5, a histone acetyltransferase, have partially redundant roles in the control of SUC2 transcription, suggesting a functional overlap between two different mechanisms believed to overcome repression by nucleosomes, nucleosome remodeling and histone acetylation.
Snf/Swi是一种核小体重塑复合体，对于酿酒酵母中克服核小体介导的转录抑制作用至关重要。我们研究了Snf/Swi在体内控制Snf/Swi依赖性启动子（即SUC2基因启动子）转录的机制。通过单细胞分析，我们的结果表明，Snf/Swi是群体中每个细胞中SUC2基因激活水平表达所必需的。此外，Snf/Swi是维持SUC2转录所必需的，这表明持续的染色质重塑对于维持活跃的转录状态是必要的。最后，Snf/Swi和组蛋白乙酰转移酶Gcn5在控制SUC2转录方面具有部分冗余作用，这表明在两种不同的被认为可克服核小体抑制作用的机制（核小体重塑和组蛋白乙酰化）之间存在功能重叠。
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