[肿瘤坏死因子-α和巨噬细胞集落刺激因子对骨巨细胞瘤诱导的骨溶解的促进作用]
[Promotive effect of TNF-alpha and M-CSF on osteolysis induced by giant cell tumor of bone].
作者信息
Wen J, Xie D, Yao J
机构信息
Department of Pathology, Sun Yet-San University of Medical Sciences, Guangzhou.
出版信息
Zhonghua Bing Li Xue Za Zhi. 1997 Oct;26(5):273-6.
OBJECTIVE
To explore the effect of cell components in giant cell tumor of bone (GCT) and cytokines expressed by them on osteolysis.
METHODS
Mononuclear stromal cells and multinucleated giant cells (MGCs) were isolated from 10 cases of GCT, and fibroblast-like stromal cells were obtained by long-term culture of mononuclear stromal cells. Osteolytic capability of above isolated cells were tested in an in vitro cell-bone resorption model.
RESULTS
All cell components isolated from GCT had capability to resorb bone matrix directly. Exogenous tumore necrosis factor-alpha (TNF-alpha) could significantly increase the bone resorption induced by both kinds of stromal cell. There were higher level of TNF-alpha and greater expression rate of macrophage colony stimulating factor (M-CSF) in GCT than in the osteosarcoma tissues or the normal serum.
CONCLUSIONS
The characteristic bone resorption behavior of GCT might be conducted by all it's three major cell components, and this bone resorption process could be promoted by both TNF-alpha and M-CSF they expressed.
目的
探讨骨巨细胞瘤（GCT）中的细胞成分及其表达的细胞因子对骨溶解的影响。
方法
从10例GCT中分离出单核基质细胞和多核巨细胞（MGCs），通过对单核基质细胞进行长期培养获得成纤维样基质细胞。在体外细胞-骨吸收模型中检测上述分离细胞的溶骨能力。
结果
从GCT中分离出的所有细胞成分均具有直接吸收骨基质的能力。外源性肿瘤坏死因子-α（TNF-α）可显著增加两种基质细胞诱导的骨吸收。GCT中TNF-α水平高于骨肉瘤组织或正常血清，巨噬细胞集落刺激因子（M-CSF）的表达率更高。
结论
GCT特有的骨吸收行为可能由其三种主要细胞成分共同介导，且它们表达的TNF-α和M-CSF均可促进这一骨吸收过程。
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