鲷科（硬骨鱼纲，鲈形目）着丝粒卫星DNA的进化及其在系统发育研究中的应用

Evolution of centromeric satellite DNA and its use in phylogenetic studies of the Sparidae family (Pisces, Perciformes).

作者信息

Garrido-Ramos M A, de la Herrán R, Jamilena M, Lozano R, Ruiz Rejón C, Ruiz Rejón M

机构信息

Facultad de Ciencias, Universidad de Granada, Granada, 18071, Spain.

出版信息

Mol Phylogenet Evol. 1999 Jul;12(2):200-4. doi: 10.1006/mpev.1998.0609.

DOI:10.1006/mpev.1998.0609

PMID:10381322

Abstract

In this paper, we use the EcoRI centromeric satellite DNA family conserved in Sparidae as a taxonomic and a phylogenetic marker. The analyses of 56 monomeric units (187 bp in size) obtained by means of cloning and PCR from 10 sparid species indicate that this repetitive DNA evolves by concerted evolution. Different phylogenetic inference methods, such as neighbor-joining and UPGMA, group the 56 repeats by taxonomic affinity and support the existence of at least two monophyletic groups within the Sparidae family. These results reinforce the recent taxonomic revision of the genera Sparus and Pagrus and contradict previous classifications of the Sparidae family.

摘要

在本文中，我们将鲷科中保守的EcoRI着丝粒卫星DNA家族用作分类学和系统发育标记。通过克隆和PCR从10种鲷科鱼类中获得的56个单体单元（大小为187 bp）的分析表明，这种重复DNA通过协同进化而进化。不同的系统发育推断方法，如邻接法和UPGMA，根据分类学亲缘关系对这56个重复序列进行分组，并支持鲷科中至少存在两个单系类群。这些结果强化了最近对 Sparus 属和 Pagrus 属的分类修订，并与之前鲷科的分类相矛盾。