人胶质瘤中p21WAF1/CIP1表达与放射抗性的关系。
Relationship between expression of p21WAF1/CIP1 and radioresistance in human gliomas.
作者信息
Kokunai T, Tamaki N
机构信息
Department of Neurosurgery, Kobe University School of Medicine.
出版信息
Jpn J Cancer Res. 1999 Jun;90(6):638-46. doi: 10.1111/j.1349-7006.1999.tb00795.x.
Abstract
The role of p21WAF1/CIP1 (p21) in DNA repair and apoptosis following gamma-irradiation remains controversial. In this study the influence of p21 on the radiosensitivity of human brain tumors was investigated. Resected tumors were stained immunohistochemically for p21. Expression of p21 in astrocytic tumors was high, but it was low in medulloblastomas, germinomas, and primary malignant lymphomas. Glioma and medulloblastoma cell lines were transfected with pcDNA/p21 to cause p21 overexpression, then tumor-cell colony formation and apoptosis were assessed following gamma-irradiation of the transfected and nontransfected cells. Overexpression of p21 enhanced clonogenic survival and suppressed apoptosis after gamma-irradiation in human brain tumor cell lines with or without p53 protein deficiency. Radioresistance was acquired when p21 was overproduced in the glioma cell lines irrespective of p53 status.
摘要
p21WAF1/CIP1（p21）在γ射线照射后的DNA修复和细胞凋亡中的作用仍存在争议。在本研究中，研究了p21对人脑肿瘤放射敏感性的影响。对切除的肿瘤进行p21免疫组织化学染色。p21在星形细胞瘤中的表达较高，但在髓母细胞瘤、生殖细胞瘤和原发性恶性淋巴瘤中表达较低。用pcDNA/p21转染胶质瘤和髓母细胞瘤细胞系以导致p21过表达，然后在对转染和未转染的细胞进行γ射线照射后评估肿瘤细胞集落形成和细胞凋亡。在有或无p53蛋白缺陷的人脑肿瘤细胞系中，p21的过表达增强了克隆存活并抑制了γ射线照射后的细胞凋亡。无论p53状态如何，当p21在胶质瘤细胞系中过量产生时，都会获得放射抗性。
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本文引用的文献
1
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.用于多瘤病毒转化细胞选择性测定的琼脂悬浮培养
Virology. 1964 Jun;23:291-4. doi: 10.1016/0042-6822(64)90301-0.
2
Variations in several responses of HeLa cells to x-irradiation during the division cycle.在分裂周期中，海拉细胞对X射线照射的几种反应的变化。
Biophys J. 1963 Jan;3(1):11-33. doi: 10.1016/s0006-3495(63)86801-0.
3
Resistance to Fas-mediated apoptosis: activation of caspase 3 is regulated by cell cycle regulator p21WAF1 and IAP gene family ILP.对Fas介导的细胞凋亡的抗性：半胱天冬酶3的激活受细胞周期调节因子p21WAF1和凋亡抑制蛋白（IAP）基因家族ILP调控。
Oncogene. 1998 Aug 27;17(8):931-9. doi: 10.1038/sj.onc.1202021.
4
Identification of human p53 mutations with differential effects on the bax and p21 promoters using functional assays in yeast.利用酵母中的功能分析鉴定对bax和p21启动子有不同影响的人类p53突变。
Oncogene. 1998 Mar 12;16(10):1369-72. doi: 10.1038/sj.onc.1201889.
5
6
The apoptotic and transcriptional transactivation activities of p53 can be dissociated.p53的凋亡和转录反式激活活性可以被分离。
Biochem Cell Biol. 1997;75(4):351-8.
7
Mutated p21(WAF1/CIP1/SDI1) lacking CDK-inhibitory activity fails to prevent apoptosis in human colorectal carcinoma cells.缺乏细胞周期蛋白依赖性激酶抑制活性的突变型p21（WAF1/CIP1/SDI1）无法预防人类结肠癌细胞的凋亡。
Oncogene. 1998 Feb 12;16(6):705-12. doi: 10.1038/sj.onc.1201585.
8
Overexpression of p21WAF1/CIP1 induces cell differentiation and growth inhibition in a human glioma cell line.p21WAF1/CIP1的过表达在人胶质瘤细胞系中诱导细胞分化并抑制生长。
Int J Cancer. 1998 Feb 9;75(4):643-8. doi: 10.1002/(sici)1097-0215(19980209)75:4<643::aid-ijc24>3.0.co;2-8.
9
Functional analysis of trk proto-oncogene product in medulloblastoma cells.
Neurol Med Chir (Tokyo). 1996 Nov;36(11):796-804. doi: 10.2176/nmc.36.796.
10
Expression of p21WAF1/CIP1 in fetal and adult tissues: simultaneous analysis with Ki67 and p53.p21WAF1/CIP1在胎儿及成人组织中的表达：与Ki67和p53的同步分析
J Clin Pathol. 1997 Aug;50(8):645-53. doi: 10.1136/jcp.50.8.645.
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