胚胎致死性异常视觉样RNA结合蛋白调节PC12细胞中的神经突生长和tau蛋白表达。
Embryonic lethal abnormal vision-like RNA-binding proteins regulate neurite outgrowth and tau expression in PC12 cells.
作者信息
Aranda-Abreu G E, Behar L, Chung S, Furneaux H, Ginzburg I
机构信息
Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, Israel.
出版信息
J Neurosci. 1999 Aug 15;19(16):6907-17. doi: 10.1523/JNEUROSCI.19-16-06907.1999.
Abstract
The embryonic lethal abnormal vision (ELAV)-like proteins are mRNA-binding proteins that regulate mRNA stability. The neuronal members of this family are required for neuronal differentiation. We identified the binding region of purified HuD protein to a target neuronal mRNA encoding for the tau microtubule-associated protein and demonstrated an in vivo interaction between the ELAV-like protein and its target tau mRNA. We show that treatment of neuronal cells with antisense oligodeoxynucleotides directed against HuD blocks the induction of neurite outgrowth and decreases the levels of tau mRNAs, indicating that the ELAV-like proteins are required for neuronal differentiation.
摘要
胚胎致死性异常视觉（ELAV）样蛋白是调节mRNA稳定性的mRNA结合蛋白。该家族的神经元成员是神经元分化所必需的。我们鉴定了纯化的HuD蛋白与编码tau微管相关蛋白的靶神经元mRNA的结合区域，并证明了ELAV样蛋白与其靶tau mRNA之间的体内相互作用。我们表明，用针对HuD的反义寡脱氧核苷酸处理神经元细胞可阻断神经突生长的诱导并降低tau mRNA的水平，这表明ELAV样蛋白是神经元分化所必需的。
相似文献
1
Embryonic lethal abnormal vision-like RNA-binding proteins regulate neurite outgrowth and tau expression in PC12 cells.
J Neurosci. 1999 Aug 15;19(16):6907-17. doi: 10.1523/JNEUROSCI.19-16-06907.1999.
2
Cytoplasmic localization is required for the mammalian ELAV-like protein HuD to induce neuronal differentiation.
Genes Cells. 1999 Nov;4(11):667-83. doi: 10.1046/j.1365-2443.1999.00292.x.
3
The RNA-binding protein HuD is required for GAP-43 mRNA stability, GAP-43 gene expression, and PKC-dependent neurite outgrowth in PC12 cells.
Mol Biol Cell. 2000 Sep;11(9):3191-203. doi: 10.1091/mbc.11.9.3191.
4
Overexpression of HuD, but not of its truncated form HuD I+II, promotes GAP-43 gene expression and neurite outgrowth in PC12 cells in the absence of nerve growth factor.
J Neurochem. 2000 Sep;75(3):1103-14. doi: 10.1046/j.1471-4159.2000.0751103.x.
5
The insulin-like growth factor mRNA binding-protein IMP-1 and the Ras-regulatory protein G3BP associate with tau mRNA and HuD protein in differentiated P19 neuronal cells.
J Neurochem. 2004 May;89(3):613-26. doi: 10.1111/j.1471-4159.2004.02371.x.
6
The RNA binding protein HuD: rat cDNA and analysis of the alternative spliced mRNA in neuronal differentiating cell lines P19 and PC12.
Brain Res Mol Brain Res. 1996 Jan;35(1-2):285-96. doi: 10.1016/0169-328x(95)00231-g.
7
Involvement of mature tau isoforms in the stabilization of neurites in PC12 cells.
J Neurosci Res. 1991 Sep;30(1):163-71. doi: 10.1002/jnr.490300117.
8
p21(waf1) mRNA contains a conserved element in its 3'-untranslated region that is bound by the Elav-like mRNA-stabilizing proteins.
J Biol Chem. 1998 Aug 7;273(32):20511-6. doi: 10.1074/jbc.273.32.20511.
9
Visualization of translated tau protein in the axons of neuronal P19 cells and characterization of tau RNP granules.
J Cell Sci. 2002 Oct 1;115(Pt 19):3817-27. doi: 10.1242/jcs.00058.
10
Expression of HuD protein is essential for initial phase of neuronal differentiation in rat pheochromocytoma PC12 cells.
Biochem Biophys Res Commun. 1998 Mar 6;244(1):226-9. doi: 10.1006/bbrc.1998.8247.
引用本文的文献
1
The Role of Tau in Neuronal Function and Neurodegeneration.
Neurol Int. 2025 May 13;17(5):75. doi: 10.3390/neurolint17050075.
2
Phylogenomic instructed target analysis reveals ELAV complex binding to multiple optimally spaced U-rich motifs.
Nucleic Acids Res. 2024 Nov 11;52(20):12712-12726. doi: 10.1093/nar/gkae826.
3
Alzheimer's Disease: Cellular and Pharmacological Aspects.
Geriatrics (Basel). 2024 Jun 22;9(4):86. doi: 10.3390/geriatrics9040086.
4
Post-transcriptional mechanisms controlling neurogenesis and direct neuronal reprogramming.
Neural Regen Res. 2024 Sep 1;19(9):1929-1939. doi: 10.4103/1673-5374.390976. Epub 2023 Dec 15.
5
Emerging Roles for the RNA-Binding Protein HuD (ELAVL4) in Nervous System Diseases.
Int J Mol Sci. 2022 Nov 23;23(23):14606. doi: 10.3390/ijms232314606.
6
Posttranscriptional regulation of neurofilament proteins and tau in health and disease.
Brain Res Bull. 2023 Jan;192:115-127. doi: 10.1016/j.brainresbull.2022.10.017. Epub 2022 Oct 29.
7
RNA-binding protein ELAVL4/HuD ameliorates Alzheimer's disease-related molecular changes in human iPSC-derived neurons.
Prog Neurobiol. 2022 Oct;217:102316. doi: 10.1016/j.pneurobio.2022.102316. Epub 2022 Jul 14.
8
Roles of Embryonic Lethal Abnormal Vision-Like RNA Binding Proteins in Cancer and Beyond.
Front Cell Dev Biol. 2022 Apr 6;10:847761. doi: 10.3389/fcell.2022.847761. eCollection 2022.
9
PKCε Activation Restores Loss of PKCε, Manganese Superoxide Dismutase, Vascular Endothelial Growth Factor, and Microvessels in Aged and Alzheimer's Disease Hippocampus.
Front Aging Neurosci. 2022 Mar 1;14:836634. doi: 10.3389/fnagi.2022.836634. eCollection 2022.
10
Translation Initiation Regulated by RNA-Binding Protein in Mammals: The Modulation of Translation Initiation Complex by Trans-Acting Factors.
Cells. 2021 Jul 6;10(7):1711. doi: 10.3390/cells10071711.
本文引用的文献
1
Identification of 3'UTR region implicated in tau mRNA stabilization in neuronal cells.
J Mol Neurosci. 1999 Apr;12(2):131-45. doi: 10.1007/BF02736927.
2
ELAV tumor antigen, Hel-N1, increases translation of neurofilament M mRNA and induces formation of neurites in human teratocarcinoma cells.
Genes Dev. 1999 Feb 15;13(4):449-61. doi: 10.1101/gad.13.4.449.
3
p21(waf1) mRNA contains a conserved element in its 3'-untranslated region that is bound by the Elav-like mRNA-stabilizing proteins.
J Biol Chem. 1998 Aug 7;273(32):20511-6. doi: 10.1074/jbc.273.32.20511.
4
The role of elav-like genes, a conserved family encoding RNA-binding proteins, in growth and development.
Semin Cell Dev Biol. 1997 Dec;8(6):577-84. doi: 10.1006/scdb.1997.0183.
5
RNA stabilization by the AU-rich element binding protein, HuR, an ELAV protein.
EMBO J. 1998 Jun 15;17(12):3461-70. doi: 10.1093/emboj/17.12.3461.
6
Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs.
EMBO J. 1998 Jun 15;17(12):3448-60. doi: 10.1093/emboj/17.12.3448.
7
Expression of HuD protein is essential for initial phase of neuronal differentiation in rat pheochromocytoma PC12 cells.
Biochem Biophys Res Commun. 1998 Mar 6;244(1):226-9. doi: 10.1006/bbrc.1998.8247.
8
Messenger RNAs in dendrites: localization, stability, and implications for neuronal function.
Bioessays. 1998 Jan;20(1):70-8. doi: 10.1002/(SICI)1521-1878(199801)20:1<70::AID-BIES10>3.0.CO;2-5.
9
Hypoxic stabilization of vascular endothelial growth factor mRNA by the RNA-binding protein HuR.
J Biol Chem. 1998 Mar 13;273(11):6417-23. doi: 10.1074/jbc.273.11.6417.
10
Messenger ribonucleoprotein complexes containing human ELAV proteins: interactions with cytoskeleton and translational apparatus.
J Cell Sci. 1998 Jan;111 ( Pt 2):183-97. doi: 10.1242/jcs.111.2.183.
Zotero 插件