骨形态发生蛋白1通过降解BMP4拮抗剂脊索蛋白来调节非洲爪蟾早期胚胎的背腹模式形成。

Bone morphogenetic protein 1 regulates dorsal-ventral patterning in early Xenopus embryos by degrading chordin, a BMP4 antagonist.

作者信息

Wardle F C, Welch J V, Dale L

机构信息

Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.

出版信息

Mech Dev. 1999 Aug;86(1-2):75-85. doi: 10.1016/s0925-4773(99)00114-8.

DOI:10.1016/s0925-4773(99)00114-8

PMID:10446267

Abstract

Bone morphogenetic protein 1 (BMP1) is a metalloprotease that ventralises dorsal mesoderm when overexpressed in early Xenopus embryos. Here we show that Xenopus BMP1 blocks the dorsalising activity of chordin, but not noggin or DeltaxBMPR, when coexpressed in the ventral marginal zone and degrades chordin protein in vitro. We also show that a dominant-negative mutation for XBMP1 (dnBMP1) dorsalises ventral mesoderm in vivo, and blocks degradation of chordin by both XBMP1 and Xolloid, a closely related Xenopus metalloprotease, in vitro. dnBMP1 does not dorsalise ventral mesoderm in UV-irradiated embryos, demonstrating that this activity is dependent upon a functional organiser--the natural source of chordin in Xenopus gastrulae. Our results suggest that XBMP1 may regulate the availability of chordin during vertebrate embryogenesis.

摘要

骨形态发生蛋白1（BMP1）是一种金属蛋白酶，在非洲爪蟾早期胚胎中过表达时会使背侧中胚层腹侧化。在此我们表明，非洲爪蟾BMP1在腹侧边缘区共表达时会阻断脊索蛋白的背侧化活性，但不会阻断头蛋白或DeltaxBMPR的背侧化活性，并且在体外会降解脊索蛋白。我们还表明，XBMP1的显性负性突变体（dnBMP1）在体内会使腹侧中胚层背侧化，并且在体外会阻断XBMP1和Xolloid（一种密切相关的非洲爪蟾金属蛋白酶）对脊索蛋白的降解。dnBMP1在紫外线照射的胚胎中不会使腹侧中胚层背侧化，这表明该活性依赖于功能性组织者——非洲爪蟾原肠胚中脊索蛋白的天然来源。我们的结果表明，XBMP1可能在脊椎动物胚胎发育过程中调节脊索蛋白的可用性。