澳大利亚昆士兰州致倦库蚊（双翅目：蚊科）对巴马森林病毒的媒介能力，以及该物种和埃及伊蚊（双翅目：蚊科）对登革1 - 4型病毒的媒介能力。

Vector competence of Aedes notoscriptus (Diptera: Culicidae) for Barmah Forest virus and of this species and Aedes aegypti (Diptera: Culicidae) for dengue 1-4 viruses in Queensland, Australia.

作者信息

Watson T M, Kay B H

机构信息

Australian Centre for International and Tropical Health and Nutrition, University of Queensland Medical School, Herston, Australia.

出版信息

J Med Entomol. 1999 Jul;36(4):508-14. doi: 10.1093/jmedent/36.4.508.

DOI:10.1093/jmedent/36.4.508

PMID:10467781

Abstract

Aedes notoscriptus (Skuse) mosquitoes colonized from Brisbane, Queensland, Australia, were fed on blood suspensions containing either Barmah Forest (BF) virus, dengue virus type 1 (DEN-1), dengue virus type 2 (DEN-2), dengue virus type 3 (DEN-3), or dengue virus type 4 (DEN-4). Ae. notoscriptus was shown to be moderately susceptible to oral infection with BF virus, ID50 = 10(3.3) CCID50 per mosquito, with titers in infected mosquitoes peaking 10 d after infection. BF virus transmission occurred between days 5 and 12, with an average rate of 45% (range, 40-50%). For DEN 1-4, Ae. notoscriptus showed low level susceptibility to oral infection, with maximum head and thorax squash infection rates of 8, 5, 13, and 3%, respectively. Rates of infection were notably higher in a north Queensland strain of Aedes aegypti (L.) used as a positive control, with maximum thorax squash infection rates of 58, 68, 78, and 60% for DEN 1-4, respectively. On the basis of these data, it is concluded that Ae. notoscriptus could act as an efficient vector of BF in urban environments, but it is unlikely to be an important vector of DEN.

摘要

从澳大利亚昆士兰州布里斯班采集的致倦库蚊（Skuse），用含有巴马森林（BF）病毒、1型登革病毒（DEN-1）、2型登革病毒（DEN-2）、3型登革病毒（DEN-3）或4型登革病毒（DEN-4）的血液悬液进行喂养。结果表明，致倦库蚊对BF病毒的经口感染具有中等易感性，半数感染剂量（ID50）为每只蚊子10(3.3) 组织培养感染剂量50（CCID50），感染蚊子中的病毒滴度在感染后10天达到峰值。BF病毒在第5天至第12天之间传播，平均传播率为45%（范围为40%-50%）。对于DEN 1-4，致倦库蚊对经口感染表现出低水平易感性，头部和胸部挤压法检测到的最大感染率分别为8%、5%、13%和3%。作为阳性对照的北昆士兰埃及伊蚊（L.）品系的感染率明显更高，DEN 1-4的胸部挤压法检测到的最大感染率分别为58%、68%、78%和60%。基于这些数据，可以得出结论，致倦库蚊在城市环境中可能是BF的有效传播媒介，但不太可能是DEN的重要传播媒介。