Wang X, Krebs L J, Al-Nuri M, Pudavar H E, Ghosal S, Liebow C, Nagy A A, Schally A V, Prasad P N
Photonics Research Laboratory, Department of Chemistry, State University of New York, Buffalo, NY 14260, USA.
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11081-4. doi: 10.1073/pnas.96.20.11081.
Chemotherapy is commonly used in the treatment of cancers. However, the mechanism of action of many of these agents is not well understood. We present the synthesis of a two-photon fluorophore (C625) and its biological application when chemically linked to a chemotherapeutic agent (AN-152). By using two-photon laser-scanning microscopy, the drug:fluorophore conjugate can be observed directly as it interacts with receptor-positive cell lines. The results of this project visually show the receptor-mediated entry of AN-152 into the cell cytoplasm and subsequently into the nucleus. These observations will allow for better understanding of the drug's therapeutic mechanism, which is a subject of ongoing research aimed at improving present methods for cancer therapy.
化疗常用于癌症治疗。然而,这些药物中许多的作用机制尚未完全了解。我们展示了一种双光子荧光团(C625)的合成及其与化疗药物(AN - 152)化学连接后的生物学应用。通过双光子激光扫描显微镜,可以直接观察到药物:荧光团缀合物与受体阳性细胞系相互作用的情况。该项目的结果直观地显示了AN - 152通过受体介导进入细胞质并随后进入细胞核。这些观察结果将有助于更好地理解药物的治疗机制,这是正在进行的旨在改进当前癌症治疗方法的研究课题。