长期暴露于低温后不可培养的空肠弯曲螺旋菌细胞的活力和DNA维持
Viability and DNA maintenance in nonculturable spiral Campylobacter jejuni cells after long-term exposure to low temperatures.
作者信息
Lázaro B, Cárcamo J, Audícana A, Perales I, Fernández-Astorga A
机构信息
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV/EHU), 01080 Vitoria-Gasteiz, Spain.
出版信息
Appl Environ Microbiol. 1999 Oct;65(10):4677-81. doi: 10.1128/AEM.65.10.4677-4681.1999.
Abstract
Survival of Campylobacter jejuni at 4 and 20 degrees C was investigated by using cellular integrity, respiratory activity, two-dimensional (2D) protein profile, and intact DNA content as indicators of potential viability of nonculturable cells. Intact DNA content after 116 days, along with cellular integrity and respiring cells, was detected for up to 7 months at 4 degrees C by pulsed-field gel electrophoresis. Most changes in 2D protein profiles involved up- or down-regulation.
摘要
通过使用细胞完整性、呼吸活性、二维（2D）蛋白质谱和完整DNA含量作为不可培养细胞潜在活力的指标，研究了空肠弯曲菌在4℃和20℃下的存活情况。通过脉冲场凝胶电泳在4℃下长达7个月检测到116天后的完整DNA含量，以及细胞完整性和呼吸细胞。二维蛋白质谱的大多数变化涉及上调或下调。
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