大肠杆菌中的重组蛋白表达。

Recombinant protein expression in Escherichia coli.

作者信息

Baneyx F

机构信息

Department of Chemical Engineering University of Washington Box 351750, Seattle, WA 98195, USA.

出版信息

Curr Opin Biotechnol. 1999 Oct;10(5):411-21. doi: 10.1016/s0958-1669(99)00003-8.

DOI:10.1016/s0958-1669(99)00003-8

PMID:10508629

Abstract

Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, together with serendipitous discoveries and the availability of improved genetic tools are making this bacterium more valuable than ever for the expression of complex eukaryotic proteins.

摘要

大肠杆菌是生产异源蛋白最广泛使用的宿主之一，其遗传学特征比任何其他微生物都要清楚得多。近年来，人们对大肠杆菌转录、翻译和蛋白质折叠的基本理解取得了进展，加上一些意外的发现以及更先进遗传工具的出现，使得这种细菌在表达复杂真核蛋白方面比以往任何时候都更具价值。