溶液中大分子和多分子聚集体的核磁共振光谱学。

NMR spectroscopy of large molecules and multimolecular assemblies in solution.

作者信息

Wider G, Wüthrich K

机构信息

Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, CH-8093, Zürich, Switzerland.

出版信息

Curr Opin Struct Biol. 1999 Oct;9(5):594-601. doi: 10.1016/s0959-440x(99)00011-1.

DOI:10.1016/s0959-440x(99)00011-1

PMID:10508768

Abstract

New strategies and technical advances in NMR spectroscopy and biochemical methods for isotope labeling have enabled solution NMR studies of biomacromolecular systems of 100 kDa and larger. Recent progress has been made, in particular, with techniques for sequential resonance assignments, novel approaches for the direct observation of hydrogen bonds in nucleic acids and proteins, and segmental isotope labeling.

摘要

核磁共振光谱学以及同位素标记生化方法的新策略和技术进步，使得对100 kDa及更大分子量的生物大分子系统进行溶液核磁共振研究成为可能。特别是在序列共振归属技术、直接观察核酸和蛋白质中氢键的新方法以及片段同位素标记方面取得了进展。