Chizhov A O, Dell A, Morris H R, Haslam S M, McDowell R A, Shashkov A S, Nifant'ev N E, Khatuntseva E A, Usov A I
Biochemistry Department, Wolfson Laboratories, Imperial College, London, UK.
Carbohydr Res. 1999 Jul 20;320(1-2):108-19. doi: 10.1016/s0008-6215(99)00148-2.
Fucoidan fractions from the brown seaweed Chorda filum were studied using solvolytic desulfation. Methylation analysis and NMR spectroscopy were applied for native and desulfated polysaccharides. Homofucan sulfate from C. filum was shown to contain poly-alpha-(1-->3)-fucopyranoside backbone with a high degree of branching, mainly of alpha-(1-->2)-linked single units. Some fucopyranose residues are sulfated at O-4 (mainly) and O-2 positions. Some alpha-(1-->3)-linked fucose residues were shown by NMR to be 2-O-acetylated. The 1H and 13C NMR spectra of desulfated, deacetylated fucan were completely assigned. The spectral data obtained correspond to a quasiregular polysaccharide structure with a branched hexasaccharide repeating unit. Other fucoidan fractions from C. filum have more complex carbohydrate composition and give rather complex methylation patterns. [formula: see text]
利用溶剂解脱硫法对褐藻水云的岩藻依聚糖级分进行了研究。对天然多糖和脱硫多糖进行了甲基化分析和核磁共振光谱分析。结果表明，水云的硫酸同型岩藻聚糖含有高度分支的聚-α-(1→3)-岩藻糖吡喃糖苷主链，主要由α-(1→2)-连接的单糖单元组成。一些岩藻糖吡喃糖残基在O-4（主要）和O-2位被硫酸化。核磁共振显示一些α-(1→3)-连接的岩藻糖残基被2-O-乙酰化。对脱硫、脱乙酰岩藻聚糖的1H和13C核磁共振光谱进行了完全归属。获得的光谱数据对应于具有分支六糖重复单元的准规则多糖结构。水云的其他岩藻依聚糖级分具有更复杂的碳水化合物组成，并给出相当复杂的甲基化模式。[化学式：见原文]
