鲍曼不动杆菌252中多聚磷酸激酶基因表达的调控
Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252.
作者信息
Gavigan J A, Marshall L M, Dobson A D
机构信息
Microbiology Department and National Food Biotechnology Centre, National University of Ireland Cork.
出版信息
Microbiology (Reading). 1999 Oct;145 ( Pt 10):2931-7. doi: 10.1099/00221287-145-10-2931.
A strain of Acinetobacter baumannii cultured in butyric acid media was found to take up phosphate following a period of phosphate release. PCR was used to clone the polyphosphate kinase (ppk) gene from the strain. The promoter for the ppk gene was functional in the heterologous Escherichia coli host. Using RT-PCR, transcription of the ppk gene was found to be regulated by phosphate concentration.
在丁酸培养基中培养的一株鲍曼不动杆菌被发现，在一段时期的磷酸盐释放后会摄取磷酸盐。利用聚合酶链式反应（PCR）从该菌株中克隆多聚磷酸激酶（ppk）基因。ppk基因的启动子在异源宿主大肠杆菌中具有功能。通过逆转录聚合酶链式反应（RT-PCR）发现，ppk基因的转录受磷酸盐浓度调控。
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