一种微制造的荧光激活细胞分选仪。

A microfabricated fluorescence-activated cell sorter.

作者信息

Fu A Y, Spence C, Scherer A, Arnold F H, Quake S R

机构信息

Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.

出版信息

Nat Biotechnol. 1999 Nov;17(11):1109-11. doi: 10.1038/15095.

DOI:10.1038/15095

PMID:10545919

Abstract

We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (microFACS) for sorting various biological entities. Compared with conventional FACS machines, the microFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used microFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.

摘要

我们展示了一种用于分选各种生物实体的一次性微制造荧光激活细胞分选仪（微型流式细胞仪）。与传统的流式细胞仪相比，微型流式细胞仪具有更高的灵敏度、无交叉污染且成本更低。我们已使用微型流式细胞仪芯片实现了对不同颜色微米级荧光珠群体的大量富集。此外，我们从非荧光大肠杆菌细胞背景中分离出了表达绿色荧光蛋白的大肠杆菌细胞，并表明从分选装置中提取后这些细菌仍具有活力。这些分选仪既可以作为独立设备运行，也可以作为集成微分析芯片的组件。