构建具有高LukS样功能的葡萄球菌杀白细胞素潘顿-瓦伦丁组分的LukS-PV突变体。
Construction of a LukS-PV mutant of a staphylococcal Panton-Valentine leukocidin component having a high LukS-like function.
作者信息
Shimatani A, Kaneko J, Tomita T, Kamio Y
机构信息
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
出版信息
Biosci Biotechnol Biochem. 1999 Oct;63(10):1828-30. doi: 10.1271/bbb.63.1828.
A 2-residue (D12I13) segment of LukS of a staphylococcal leukocidin component is an essential region for the hemolytic function of LukS towards rabbit erythrocytes in the presence of LukF. Here, we report that insertion of D, I, or AA residue(s) between A11 and E12 residues of LukS-PV, in which the 2-residue D12I13 segment in LukS was absent, confers the full LukS function on LukS-PV, which has only 4% hemolytic activity of that of LukS towards rabbit erythrocytes.
葡萄球菌杀白细胞素成分LukS的一个二残基（D12I13）片段是LukS在LukF存在时对兔红细胞溶血功能的关键区域。在此，我们报道，在LukS-PV的A11和E12残基之间插入D、I或AA残基（LukS中不存在二残基D12I13片段），可赋予LukS-PV完整的LukS功能，LukS-PV对兔红细胞的溶血活性仅为LukS的4%。
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