通过随机扩增多态性DNA分析确定的鲑肾杆菌分离株的分子多样性。
Molecular diversity of Renibacterium salmoninarum isolates determined by randomly amplified polymorphic DNA analysis.
作者信息
Grayson T H, Atienzar F A, Alexander S M, Cooper L F, Gilpin M L
机构信息
Department of Biological Sciences, University of Plymouth, Plymouth PL4 8AA, Devon, United Kingdom.
出版信息
Appl Environ Microbiol. 2000 Jan;66(1):435-8. doi: 10.1128/AEM.66.1.435-438.2000.
Abstract
The molecular diversity among 60 isolates of Renibacterium salmoninarum which differ in place and date of isolation was investigated by using randomly amplified polymorphic DNA (RAPD) analysis. Isolates were grouped into 21 banding patterns which did not reflect the biological source. Four 16S-23S rRNA intergenic spacer (ITS1) sequence variations and two alleles of an exact tandem repeat locus, ETR-A, were the bases for formation of distinct groups within the RAPD clusters. This study provides evidence that the most common ITS1 sequence variant, SV1, possesses two copies of a 51-bp repeat unit at ETR-A and has been widely dispersed among countries which are associated with mainstream intensive salmonid culture.
摘要
利用随机扩增多态性DNA（RAPD）分析，对60株分离地点和日期不同的鲑肾杆菌进行了分子多样性研究。分离株被分为21种条带模式，这些模式并未反映其生物学来源。四个16S - 23S rRNA基因间隔区（ITS1）序列变异以及一个精确串联重复位点ETR - A的两个等位基因，是在RAPD聚类中形成不同组别的基础。本研究提供了证据，表明最常见的ITS1序列变异体SV1在ETR - A处拥有两个51碱基重复单元的拷贝，并且已在与主流集约化鲑鱼养殖相关的国家中广泛传播。
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