利用线粒体DNA分析对佩德罗霉样真菌进行分子流行病学研究。

Molecular epidemiology of Fonsecaea pedrosoi using mitochondrial DNA analysis.

作者信息

Kawasaki M, Aoki M, Ishizaki H, Miyaji M, Nishimura K, Nishimoto K, Matsumoto T, De Vroey C, Negroni R, Mendonca M, Andriantsimahavandy A, Esterre P

机构信息

Department of Dermatology, Kanazawa Medical University, Ishikawa, Japan.

出版信息

Med Mycol. 1999 Dec;37(6):435-40.

PMID:10647125

Abstract

Restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA) from 49 clinical Fonsecaea pedrosoi isolates (18 isolates from Japan, 17 from Madagascar, 7 from Argentina, 5 from Venezuela, 1 from Costa Rica and 1 unknown) was studied. The 49 isolates were composed of 20 isolates of Type 1, 16 of Type 2, 12 of Type 4 and 1 of a new mtDNA type, Type 7, which was closely related to Type 2. On the bases of the results of 120 isolates of the present (49 isolates) and previous (71 isolates) studies, F. pedrosoi was classified into seven mtDNA types and according to the relationship between mtDNA types and geographic origins: in Japan and probably in China, Type 1 isolates; in Zaire and Madagascar, Type 2; in Central and South America, Type 4 and Type 1. These results indicated that the geographical origin of F. pedrosoi isolate could be roughly inferred from its mtDNA type.

摘要

对49株临床分离的佩德罗分支孢子菌线粒体DNA（mtDNA）的限制性片段长度多态性（RFLP）进行了研究。这49株分离株来自日本（18株）、马达加斯加（17株）、阿根廷（7株）、委内瑞拉（5株）、哥斯达黎加（1株）以及1株来源不明。49株分离株包括20株1型、16株2型、12株4型以及1株新的mtDNA类型，即7型，它与2型密切相关。根据本次研究的49株以及之前研究的71株共120株分离株的结果，佩德罗分支孢子菌被分为7种mtDNA类型，并根据mtDNA类型与地理来源之间的关系进行划分：在日本以及可能在中国，为1型分离株；在扎伊尔和马达加斯加，为2型；在中美洲和南美洲，为4型和1型。这些结果表明，佩德罗分支孢子菌分离株的地理来源可根据其mtDNA类型大致推断。