用于高效评估高度微卫星不稳定结直肠癌肿瘤的微卫星标记物评估

Evaluation of microsatellite markers for efficient assessment of high microsatellite instabile colorectal tumors.

作者信息

Potocnik U, Glavac D, Golouh R, Ravnik-Glavac M

机构信息

Laboratory of Molecular Genetics, Institute of Pathology, Medical Faculty, Ljubljana, Slovenia.

出版信息

Pflugers Arch. 2000;439(3 Suppl):R47-9.

PMID:10653139

Abstract

Two hundred thirty randomly collected primary colorectal tumors were initially screened for microsatellite instability (MSI) with three highly informative microsatellite markers (BAT26, D2S123 and D5S346). Forty one (17.8%) tumors showed alterations in at least one marker. In further MSI analysis of these 41 MSI tumors with additional 9 markers, 21 tumors (9.6% of 230 analyzed) exhibited MSI at more than 40% and the rest 20 (8.7% of 230 analyzed) tumors exhibited MSI at 8%-20% tested markers. These results support classification of MSI tumors into high MSI tumors (more than 40% unstable loci) and low MSI tumors (less than 20% unstable loci). Based on our results the combination of BAT26 and two out of four other highly informative markers (D2S123, D5S346, BAT25 or BAT40) is recommended for rapid and reliable assessment of high MSI tumors.

摘要

使用三个信息丰富的微卫星标记（BAT26、D2S123和D5S346）对230个随机收集的原发性结直肠癌肿瘤进行微卫星不稳定性（MSI）初步筛查。41个（17.8%）肿瘤至少在一个标记中显示改变。在对这41个MSI肿瘤使用另外9个标记进行的进一步MSI分析中，21个肿瘤（占分析的230个肿瘤的9.6%）显示MSI超过40%，其余20个（占分析的230个肿瘤的8.7%）肿瘤在8%-20%的检测标记中显示MSI。这些结果支持将MSI肿瘤分为高MSI肿瘤（不稳定位点超过40%）和低MSI肿瘤（不稳定位点少于20%）。基于我们的结果，推荐使用BAT26与其他四个信息丰富的标记（D2S123、D5S346、BAT25或BAT40）中的两个进行组合，以快速可靠地评估高MSI肿瘤。