Holdcroft A, Bose D, Sapsed-Byrne S M, Ma D, Lockwood G G
Department of Anaesthesia, Imperial College School of Medicine, Hammersmith Hospital, London, UK.
Br J Anaesth. 1999 Oct;83(4):618-21. doi: 10.1093/bja/83.4.618.
The inspired partial pressure of an anaesthetic is often used as an index of arterial partial pressure in small animal experiments. We have investigated the influence of anaesthetic solubility on the ratio of arterial to inspired partial pressure in 24 rats, allocated randomly to receive halothane, isoflurane or desflurane at four different inspired concentrations. The arterial partial pressure of the volatile agent was measured by two-stage headspace analysis using a gas chromatograph calibrated with the same gas used to calibrate the Datex Capnomac that measured the inspired concentration. Mean values of arterial to inspired ratio at the lowest concentrations were 0.60 (95% confidence intervals 0.50, 0.71) for 0.8% halothane, 0.54 (0.38, 0.69) for 0.8% isoflurane, 0.72 (0.59, 0.86) for 1.5% sevoflurane and 0.71 (0.54, 0.87) for 4% desflurane. Analysis of variance showed a significant effect of anaesthetic agent (P = 0.008) on the arterial to inspired ratio. Thus volatile anaesthetic agents do not demonstrate a fixed arterial to inspired ratio in rats.
在小动物实验中，麻醉药的吸入分压常被用作动脉分压的指标。我们研究了麻醉药溶解度对24只大鼠动脉分压与吸入分压比值的影响，这些大鼠被随机分配接受四种不同吸入浓度的氟烷、异氟烷或地氟烷。挥发性麻醉药的动脉分压通过两阶段顶空分析法测量，使用的气相色谱仪用与校准测量吸入浓度的Datex Capnomac相同的气体进行校准。最低浓度下动脉与吸入比值的平均值，0.8%氟烷为0.60（95%置信区间0.50，0.71），0.8%异氟烷为0.54（0.38，0.69），1.5%七氟烷为0.72（0.59，0.86），4%地氟烷为0.71（0.54，0.87）。方差分析显示麻醉药对动脉与吸入比值有显著影响（P = 0.008）。因此，挥发性麻醉药在大鼠中并未表现出固定的动脉与吸入比值。
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