Monteiro F A, Pérez R, Panzera F, Dujardin J P, Galvão C, Rocha D, Noireau F, Schofield C, Beard C B
Centers for Disease Control and Prevention, Atlanta, GA, USA.
Mem Inst Oswaldo Cruz. 1999;94 Suppl 1:229-38. doi: 10.1590/s0074-02761999000700037.
DNA sequence comparison of 412 base-pairs fragments of the mitochondrial cytochrome B gene was used to infer the genetic structure of nine geographical Triatoma infestans populations and their phylogenetic relationship with T. melanosoma and T. brasiliensis. T. infestans and T. melanosoma were compared by morphometry, allozyme and cytogenetic analyses, as well as subjected to reciprocal crosses, in order to clarify the taxonomic status of the latter. No differences were found to distinguish the two species and the crosses between them yielded progeny. T. infestans populations presented four haplotypes that could be separated in two clusters: one formed by the samples from Bolivia (Andes and Chaco) and the other formed by samples from Argentina and Brazil. Silvatic and domestic T. infestans populations from Bolivia (Andes) were genetically identical.
线粒体细胞色素B基因412个碱基对片段的DNA序列比较被用于推断九个地理区域的侵袭锥蝽种群的遗传结构,以及它们与黑锥蝽和巴西锥蝽的系统发育关系。通过形态测量、等位酶和细胞遗传学分析对侵袭锥蝽和黑锥蝽进行比较,并进行正反交,以阐明后者的分类地位。未发现区分这两个物种的差异,它们之间的杂交产生了后代。侵袭锥蝽种群呈现出四种单倍型,可分为两个簇:一个由来自玻利维亚(安第斯山脉和查科)的样本组成,另一个由来自阿根廷和巴西的样本组成。来自玻利维亚(安第斯山脉)的野生和家养侵袭锥蝽种群在基因上是相同的。