123I-白细胞介素-2闪烁扫描术用于克罗恩病肠道单核细胞浸润的体内评估

123I-interleukin-2 scintigraphy for in vivo assessment of intestinal mononuclear cell infiltration in Crohn's disease.

作者信息

Signore A, Chianelli M, Annovazzi A, Bonanno E, Spagnoli L G, Pozzilli P, Pallone F, Biancone L

机构信息

Department of Clinical Sciences, University La Sapienza, Rome, Italy.

出版信息

J Nucl Med. 2000 Feb;41(2):242-9.

PMID:10688106

Abstract

UNLABELLED

Activated mononuclear cells expressing interleukin-2 (IL2) receptors (IL2-Rs) heavily infiltrate the Crohn's disease (CD) gut wall. A new technique for the in vivo detection of tissue infiltrating IL2-R positive (IL2R+ve) cells was developed based on 123I-IL2 scintigraphy. The aim of this study was to investigate whether 123I-IL2 accumulates in the CD gut wall in different phases of the disease and to evaluate the specificity of 123I-IL2 binding to activated IL2R+ve cells infiltrating the gut wall.

METHODS

Fifteen patients with ileal CD (10 active and 5 inactive) and 10 healthy volunteers were studied by 123I-IL2 scintigraphy. Six patients with active CD were studied before and after 12 wk of steroid treatment. After scintigraphy, patients were followed up for 29-54 mo. Ex vivo autoradiography was performed to determine specificity of 125I-IL2 binding to IL2R+ve cells. For bowel scintigraphy, 123I-IL2 (75 MBq) was injected intravenously and gamma camera images were acquired after 1 h. Bowel radioactivity was quantified in 64 regions of interest (ROIs).

RESULTS

Autoradiography showed specific binding of 125I-IL2 to IL2R+ve mononuclear cells infiltrating the CD gut wall. Intestinal 123I-IL2 uptake assessed by the number of positive ROIs was higher in patients with active or inactive CD than in healthy volunteers (P < 0.0001 and P = 0.03, respectively) and positively correlated with the CD activity index (P = 0.01). 123I-IL2 intestinal uptake significantly decreased in patients with CD in steroid-induced remission (P = 0.03). A significant correlation was observed between the number of positive ROIs and time to disease relapse.

CONCLUSION

123I-IL2 accumulates in the diseased CD gut wall by specific binding to IL2R+ve cells, infiltrating the involved tissues. 123I-IL2 scintigraphy may be an objective tool for the in vivo assessment of intestinal activated mononuclear cell infiltration.

摘要

未标记

表达白细胞介素-2（IL2）受体（IL2-R）的活化单核细胞大量浸润克罗恩病（CD）的肠壁。基于123I-IL2闪烁扫描技术开发了一种体内检测组织浸润性IL2-R阳性（IL2R+ve）细胞的新技术。本研究的目的是调查123I-IL2在疾病不同阶段是否在CD肠壁中蓄积，并评估123I-IL2与浸润肠壁的活化IL2R+ve细胞结合的特异性。

方法

对15例回肠CD患者（10例活动期和5例非活动期）和10名健康志愿者进行123I-IL2闪烁扫描。对6例活动期CD患者在类固醇治疗12周前后进行研究。闪烁扫描后，对患者进行29 - 54个月的随访。进行体外放射自显影以确定125I-IL2与IL2R+ve细胞结合的特异性。对于肠道闪烁扫描，静脉注射123I-IL2（75 MBq），1小时后采集γ相机图像。在64个感兴趣区域（ROI）对肠道放射性进行定量。

结果

放射自显影显示125I-IL2与浸润CD肠壁的IL2R+ve单核细胞特异性结合。通过阳性ROI数量评估的肠道123I-IL2摄取在活动期或非活动期CD患者中高于健康志愿者（分别为P < 0.0001和P = 0.03），且与CD活动指数呈正相关（P = 0.01）。类固醇诱导缓解的CD患者中123I-IL2肠道摄取显著降低（P = 0.03）。阳性ROI数量与疾病复发时间之间存在显著相关性。