牛心水病病原体反刍动物考德里氏体在蜱细胞系中的生长
Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line.
作者信息
Bell-Sakyi L, Paxton E A, Munderloh U G, Sumption K J
机构信息
Centre for Tropical Veterinary Medicine, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
出版信息
J Clin Microbiol. 2000 Mar;38(3):1238-40. doi: 10.1128/JCM.38.3.1238-1240.2000.
Abstract
The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.
摘要
蜱传立克次氏体反刍兽考德里氏体，通过使用从牛内皮细胞分离的加德尔毒株作为接种物，已在肩胛硬蜱蜱细胞系IDE8中连续传代500多天。通过聚合酶链反应、核型分析、电子显微镜检查以及牛细胞再感染，证实了蜱细胞的感染。
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