Ghosh P K, Gupta S, Ghosh R, Ruiz B H, Ortiz-Ortiz L
Departmento de Inmunologia, Instituto de Investigaciones Biomedicas UNAM, Mexico DF.
Indian J Exp Biol. 1999 Aug;37(8):803-6.
The reactivity of sera from experimentally infected animal was studied from 5-60 days postinoculation to determine which of the E. histolytica antigens are recognized frequently to infection. Crude extract of E. histolytica trophozoites was used and sera were examined by immunoelectrotransference assay. It was observed that sera recognized polypeptide with 70 kDa molecular mass after 15 days postinoculation onward and later 14 to 24 polypeptide with molecular weight of 110-22 kDa were recognized. All the amebic antigens (polypeptides) could be recognized by sera till 60 days postinoculated animals. Significance of expression of different amebic polypeptides in terms of pathogenesis needs further investigations.
对实验感染动物接种后5至60天的血清反应性进行了研究，以确定溶组织内阿米巴的哪些抗原经常被感染所识别。使用溶组织内阿米巴滋养体的粗提物，并通过免疫电转移测定法检测血清。观察到接种后15天起血清可识别分子量为70 kDa的多肽，随后可识别分子量为110 - 22 kDa的14至24种多肽。在接种后60天内，所有阿米巴抗原（多肽）均可被血清识别。不同阿米巴多肽在发病机制方面表达的意义需要进一步研究。
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