关于锌（II）离子通过大肠杆菌中坑状无机磷酸盐转运系统进行转运的证据。

Evidence for the transport of zinc(II) ions via the pit inorganic phosphate transport system in Escherichia coli.

作者信息

Beard S J, Hashim R, Wu G, Binet M R, Hughes M N, Poole R K

机构信息

Division of Life Sciences, King's College London, Campden Hill Road, London, UK.

出版信息

FEMS Microbiol Lett. 2000 Mar 15;184(2):231-5. doi: 10.1111/j.1574-6968.2000.tb09019.x.

DOI:10.1111/j.1574-6968.2000.tb09019.x

PMID:10713426

Abstract

A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit). The pitA mutant accumulated reduced amounts of zinc(II) when exposed to 0.5-2.0 mM ZnSO(4) during growth in Luria-Bertani medium.

摘要

通过转座子（Tn10dCam）诱变产生抗锌（II）突变体，鉴定出大肠杆菌K-12中一个参与锌（II）摄取的基因座。该突变位于pitA基因内，pitA基因编码低亲和力无机磷酸盐转运系统（Pit）。在Luria-Bertani培养基中生长时，当暴露于0.5-2.0 mM ZnSO₄时，pitA突变体积累的锌（II）量减少。