通过5-乙烯基脱氧尿苷实现DNA的可逆光连接
Reversible photoligation of DNA via 5-vinyldeoxyuridine.
作者信息
Fujimoto K, Matsuda S, Saito I
机构信息
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Japan.
出版信息
Nucleic Acids Symp Ser. 1999(42):39-40. doi: 10.1093/nass/42.1.39.
We report a novel non-enzymatic template-directed reversible photoligation of oligodeoxyribonucleotides (ODNs). In this photoligation system, a modified ODN containing 5-vinyldeoxyuridine at 5'-end reacts by photoirradiation at 366 nm with cytosine and thymine at the 3'-end of another ODN in the presence of a complementary template to yield a ligated ODN quantitatively. The ligated ODN can be split site-selectively to regenerate the parent ODN by photoirradiation at 302 nm.
我们报道了一种新型的非酶模板导向的寡脱氧核糖核苷酸（ODN）可逆光连接反应。在该光连接体系中，一个5'-端含有5-乙烯基脱氧尿苷的修饰ODN，在互补模板存在的情况下，于366 nm进行光照射时，会与另一个ODN 3'-端的胞嘧啶和胸腺嘧啶发生反应，从而定量生成连接的ODN。通过在302 nm进行光照射，连接的ODN可以位点选择性地裂解，以再生亲本ODN。
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