Malacinski G M, Benford H, Chung H M
J Exp Zool. 1975 Jan;191(1):97-110. doi: 10.1002/jez.1401910110.
Drastic alterations in neural morphogenesis can be induced by ultraviolet irradiation of the amphibian egg. The target area of U.V. was determined either by direct irradiation of various regions of the egg or by comparing the area of the U.V. hit with the location of the dorsal lip. Axolotl eggs which displayed easily recognizable gray crescents were employed for the direct irradiation experiments and albino axolotl eggs were used for a comparison of the U.V. sensitivity of the animal and vegetal halves of the egg. Marked frog eggs were employed to determine the relationship between the sensitive area and the site of formation of the dorsal lip. It was concluded that the dorsal marginal zone of the fertilized, uncleaved egg was the most sensitive area of the egg. Ovarian homogenates provide a source of material for correcting, by microinjection, the effects of U.V.
两栖类卵的紫外线照射可诱导神经形态发生的剧烈改变。紫外线的靶区域可通过直接照射卵的不同区域来确定，也可通过比较紫外线照射区域与背唇位置来确定。用于直接照射实验的是易于识别灰色新月区的美西螈卵，而白化美西螈卵则用于比较卵的动物半球和植物半球对紫外线的敏感性。使用有标记的蛙卵来确定敏感区域与背唇形成部位之间的关系。得出的结论是，受精未分裂卵的背侧边缘区是卵最敏感的区域。卵巢匀浆为通过显微注射纠正紫外线的影响提供了物质来源。
