Smith L H, Grohmann K, Sinsheimer R L
Nucleic Acids Res. 1974 Nov;1(11):1521-9. doi: 10.1093/nar/1.11.1521.
When using phiX174 RFI DNA as a template, invitro, E. coli RNA polymerase synthesizes four major purine triphosphate-containing 5' end sequences. RNase A digests of alpha(32)P labeled RNA were further digested with spleen exonuclease to remove the bulk of the oligonucleotides with 5' hydroxyls and then chromatographed on DEAE cellulose to resolve the remaining 5' terminal oligonucleotides. By application of standard separation and sequence techniques, the major 5' end sequences were shown to be: pppApUp(Cp), pppApApApUp(Cp), pppApApApApUp(Cp), and pppGpApUp(Gp).
当使用φX174 RFI DNA作为模板进行体外反应时，大肠杆菌RNA聚合酶会合成四种主要的含嘌呤三磷酸的5'端序列。用α(32)P标记的RNA经核糖核酸酶A消化后，再用脾外切核酸酶进一步消化，以去除大部分带有5'羟基的寡核苷酸，然后在DEAE纤维素上进行层析，以分离剩余的5'末端寡核苷酸。通过应用标准的分离和测序技术，发现主要的5'端序列为：pppApUp(Cp)、pppApApApUp(Cp)、pppApApApApUp(Cp)和pppGpApUp(Gp)。
Zotero 插件
