脱氧核苷酸取代：一种用于RNA序列分析的新技术。

Deoxynucleotide substitution: a new technique for sequence analysis of RNA.

作者信息

Van de Voorde A, Rogiers R, Van Herreweghe J, Van Heuverswyn H, Volckaert G, Fiers W

出版信息

Nucleic Acids Res. 1974 Aug;1(8):1059-67. doi: 10.1093/nar/1.8.1059.

DOI:10.1093/nar/1.8.1059

PMID:10793735

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343411/

Abstract

It is possible to replace in a normal transcription reaction catalyzed by E.coli RNA polymerase one of the four precursors by the corresponding deoxynucleoside triphosphate. These deoxynucleotide-substituted RNA's offer interesting prospects for nucleotide sequence analysis. Indeed by the use of U(2)-RNase with dG-RNA, or pancreatic RNase with dC-RNA or dU-RNA, base specific cleavage can be obtained at any of the four residues. In this way overlap of at least six residues in length can be obtained for any site in the RNA. The technique offers also great benefit for solving the sequence of the more difficult T(1)-oligonucleotides. Some examples in the sequence analysis of SV40 DNA-Hind fragments are reported.

摘要

在由大肠杆菌RNA聚合酶催化的正常转录反应中，用相应的脱氧核苷三磷酸取代四种前体之一是可能的。这些脱氧核苷酸取代的RNA为核苷酸序列分析提供了有趣的前景。实际上，通过使用U(2)-核糖核酸酶处理dG-RNA，或用胰核糖核酸酶处理dC-RNA或dU-RNA，可以在四个残基中的任何一个处获得碱基特异性切割。通过这种方式，对于RNA中的任何位点都可以获得至少六个残基长度的重叠。该技术在解决更困难的T(1)-寡核苷酸序列方面也有很大帮助。报道了SV40 DNA-Hind片段序列分析中的一些例子。