[Experimental study of apoptosis induced by cisplatin in human ovarian carcinoma AO 10/17 cells].

OBJECTIVE

Whether apoptosis induced by cisplatin resulted in tumor growth inhibition and the relationships between apoptosis and cell cycle, intracellular and extracellular Ca++ concentration were investi- gated.

METHODS

AO 10/17 cells were treated with cisplatin. Morphological changes (HE stain) were observed under light microscope; DNA degradation was assayed by electrophoresis on agarose gel. The change of cell cycle was analyzed by flow cytometer and cytosolic Ca++ concentrations were monitored using probe of calcium ion fluo-3/AM.

RESULTS

Under the influence of cisplatin, AO 10/17 cells exhibited changes of apotosis both in morphology and in the characteristics of electrophoresis. Meanwhile the cell cycle also showed special change and intracellular Ca++ concentration was increased. Increase in extracellular Ca++ concentration when AO 10/17 cells were treated with CaCl2 and cisplatin did not enhance the efficacy of cisplatin.

CONCLUSION

The results suggest that apoptosis followed by change of cytosolic Ca++ concentration is one of the mechanism of cisplatin in inhibition of ovarian tumor growth. It is closely related to cell cycle.