RanGTP结合蛋白NXT1在体外促进不同种类RNA的核输出。
RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro.
作者信息
Ossareh-Nazari B, Maison C, Black B E, Lévesque L, Paschal B M, Dargemont C
机构信息
Laboratoire de Transport Nucléocytoplasmique, Unité Mixte de Recherche 144, Institut Curie-CNRS, 75248 Paris Cedex 05, France.
出版信息
Mol Cell Biol. 2000 Jul;20(13):4562-71. doi: 10.1128/MCB.20.13.4562-4571.2000.
Abstract
To better characterize the mechanisms responsible for RNA export from the nucleus, we developed an in vitro assay based on the use of permeabilized HeLa cells. This new assay supports nuclear export of U1 snRNA, tRNA, and mRNA in an energy- and Xenopus extract-dependent manner. U1 snRNA export requires a 5' monomethylated cap structure, the nuclear export signal receptor CRM1, and the small GTPase Ran. In contrast, mRNA export does not require the participation of CRM1. We show here that NXT1, an NTF2-related protein that binds directly to RanGTP, strongly stimulates export of U1 snRNA, tRNA, and mRNA. The ability of NXT1 to promote export is dependent on its capacity to bind RanGTP. These results support the emerging view that NXT1 is a general export factor, functioning on both CRM1-dependent and CRM1-independent pathways of RNA export.
摘要
为了更好地描述细胞核中RNA输出的机制，我们基于通透化的HeLa细胞开发了一种体外检测方法。这种新的检测方法以依赖能量和非洲爪蟾提取物的方式支持U1 snRNA、tRNA和mRNA的核输出。U1 snRNA输出需要5'单甲基化帽结构、核输出信号受体CRM1和小GTP酶Ran。相比之下，mRNA输出不需要CRM1的参与。我们在此表明，NXT1是一种与NTF2相关的蛋白质，可直接与RanGTP结合，它能强烈刺激U1 snRNA、tRNA和mRNA的输出。NXT1促进输出的能力取决于其结合RanGTP的能力。这些结果支持了一种新出现的观点，即NXT1是一种通用输出因子，在RNA输出的CRM1依赖和CRM1非依赖途径中均发挥作用。
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A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export.RanBP1在核输出的最后一步中从核孔复合体释放CRM1的过程中发挥作用。
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