Cytokine responses to Der p 1 and Der p 7: house dust mite allergens with different IgE-binding activities.

BACKGROUND

There is very limited information comparing T-cell responses to different house dust mite (HDM) allergens even though T cells are essential in the initiation and regulation of immunoglobulin (Ig) E synthesis and eosinophilia.

OBJECTIVE

To compare the level of T-cell proliferation and cytokine production to the group 1 and group 7 HDM allergens which are known to have different IgE-binding capabilities.

METHODS

Freshly isolated peripheral blood mononuclear cells (PBMC) from HDM-allergic and HDM-nonallergic donors were stimulated with the group 1 and group 7 allergens of Dermatophagoides pteronyssinus and the level of proliferation as well as IL-5 and IFNgamma production were measured.

RESULTS

The proliferative and IL-5 production to the group 1 and group 7 allergens were equivalent despite the group 7 allergen's lower frequency of IgE-binding. However more IFNgamma was produced to Der p 7 than to Der p 1, particularly for the nonallergic donors. As expected IL-5 production was much higher for PBMC from the allergic donors than for cells from nonallergics; however, there was no difference in the level of T-cell proliferation between the donor groups.

CONCLUSION

The relative importance of the individual HDM allergens is normally determined by measuring the frequency of IgE-binding to the allergen in sera from an allergic population. The equivalent increased IL-5 response of PBMC from allergic people to the group 1 and group 7 allergens despite the different IgE-inducing activity indicates that these allergens may be equally capable of contributing to an asthmatic response by inducing eosinophilia.