通过六个微卫星基因座进行亲子鉴定的群体研究及验证

Population studies and validation of paternity determinations by six microsatellite loci.

作者信息

Ingvarsson S, Finnsdottir V, Sigurdsson A, Geirsson G

机构信息

Department of Pathology, University Hospital of Iceland, Reykjavik.

出版信息

J Forensic Sci. 2000 May;45(3):692-5.

PMID:10855981

Abstract

A single locus system of 6 microsatellite markers was evaluated for paternity testing. A nonradioactive method based on peroxidase labeling of a DNA probe was used to estimate the allele frequency of markers D1S216, D3S1217, D7S480, D9S157, D13S153, and D16S422 by genotyping 1134-1698 chromosomes. The number of detected alleles were 22, 15, 23, 10, 16, and 19, respectively, and the allele frequency varied from 0.001 to 0.317. The genotype of 87 families, consisting of mother, father, and child was determined. The probability that a random individual will give a positive paternity was evaluated. We conclude that the markers can be reliably typed and give sufficient and reliable information for paternity testing.

摘要

对一个由6个微卫星标记组成的单基因座系统进行了亲子鉴定评估。采用基于DNA探针过氧化物酶标记的非放射性方法，通过对1134 - 1698条染色体进行基因分型，来估计标记D1S216、D3S1217、D7S480、D9S157、D13S153和D16S422的等位基因频率。检测到的等位基因数量分别为22、15、23、10、16和19个，等位基因频率在0.001至0.317之间变化。确定了由母亲、父亲和孩子组成的87个家庭的基因型。评估了随机个体给出阳性亲子鉴定结果的概率。我们得出结论，这些标记可以可靠地分型，并为亲子鉴定提供充分且可靠的信息。