沙眼衣原体在细胞培养中的中和作用。
Neutralization of Chlamydia trachomatis in cell culture.
作者信息
Howard L V
出版信息
Infect Immun. 1975 Apr;11(4):698-703. doi: 10.1128/iai.11.4.698-703.1975.
Abstract
Neutralization of Chlamydia trachomatis was assayed by the decrease in inclusion-forming units in baby hamster kidney cells grown in culture. Five percent fresh guinea pig sera increased neutralization titers of rabbit antisera 100- to 1,000-fold but had no effect when normal rabbit sera were tested. Neutralization of a type A or B trachoma isolate was strain specific. Neutralization by human eye secretions and sera also was demonstrated when guinea pig sera were included in the test. All of the six human sera tested showed strain specificity against types A or B, in agreement with typing by the fluorescent antibody technique.
摘要
通过培养的幼仓鼠肾细胞中包涵体形成单位的减少来检测沙眼衣原体的中和作用。5%的新鲜豚鼠血清可使兔抗血清的中和效价提高100至1000倍，但检测正常兔血清时则无此作用。A或B型沙眼分离株的中和作用具有菌株特异性。当试验中加入豚鼠血清时，人眼分泌物和血清的中和作用也得到了证实。所检测的6份人血清均显示出针对A或B型的菌株特异性，这与荧光抗体技术分型结果一致。
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