[子宫内膜异位症患者的卵泡液在半透明带试验中降低了人类精子与透明带的结合]

[Follicular fluid from patients with endometriosis decreases the binding of human sperm to zona pellucida in hemizona assay].

作者信息

Qiao J, Yang S, Yao Y

机构信息

Beijing Department of Obstetrics and Gynecology, 3rd Hospital, Beijing Medical University.

出版信息

Zhonghua Yi Xue Za Zhi. 1998 Feb;78(2):98-100.

PMID:10923416

Abstract

OBJECTIVE

To evaluate the effects of follicular fluid (FF) from women with endometriosis and tubal factor on sperm binding to zona pellucida under hemizona assay(HZA).

METHODS

Follicular fluid was collected at IVF from patients with endometriosis and without endometriosis (tubal factor infertility). The HZA was used to study the effect of FF from patients with endometriosis and tubal factor on sperm binding to zona pellucida using media (EBSS/BSA) as control.

RESULTS

The mean number of sperm binding to zona in endometriosis specimens and tubal factor infertility was significantly fewer than the number treated by culture medium (91 +/- 21 vs 239 +/- 35, P < 0.001; 109 +/- 22 vs 211 +/- 42, P < 0.005). The hemizona index was lower when the incubation was performed with FF from endometriosis patients compared with sperm incubated in FF from tubal factor patients (36 +/- 4 vs 54 +/- 4, P < 0.005).

CONCLUSIONS

The inhibiting effect of FF from endometriosis patients is stronger than those without endometriosis. The follicular fluid of patients with endometriosis could contribute to infertility through impairment of gamete interaction.

摘要

目的

在半透明带试验（HZA）下，评估子宫内膜异位症和输卵管因素患者的卵泡液（FF）对精子与透明带结合的影响。

方法

在体外受精时收集子宫内膜异位症患者和非子宫内膜异位症患者（输卵管因素不孕）的卵泡液。以培养基（EBSS/BSA）作为对照，采用HZA研究子宫内膜异位症和输卵管因素患者的卵泡液对精子与透明带结合的影响。

结果

子宫内膜异位症标本和输卵管因素不孕患者中，精子与透明带结合的平均数量显著少于用培养基处理的数量（91±21对239±35，P<0.001；109±22对211±42，P<0.005）。与在输卵管因素患者卵泡液中孵育的精子相比，用子宫内膜异位症患者卵泡液孵育时半透明带指数更低（36±4对54±4，P<0.005）。