Keckeis K, Sarker S D, Dinan L N
Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, EX4 4PS Exeter, UK.
Fitoterapia. 2000 Aug;71(4):456-8. doi: 10.1016/s0367-326x(99)00159-8.
A bioassay for ecdysteroid agonists/antagonists and ecdysteroid-specific radioimmunoassays, together with a photo-diode-array detector-monitored high-performance liquid chromatography, have been used to analyse a methanol extract of the seeds of Atriplex nummularia. This yielded two phytoecdysteroids, 20-hydroxyecdysone (1) and polypodine B (2).