Ozawa K, Tsapin A I, Nealson K H, Cusanovich M A, Akutsu H
Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University, Hodogaya-ku, Yokohama 240-8501, Japan.
Appl Environ Microbiol. 2000 Sep;66(9):4168-71. doi: 10.1128/AEM.66.9.4168-4171.2000.
Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cytochrome c(3) gene from D. vulgaris Miyazaki F was used for transformation of S. oneidensis MR-1, resulting in the production of protein product that was indistinguishable from that produced by D. vulgaris Miyazaki F, except for the presence of one extra alanine residue at the N terminus.
来自普通脱硫弧菌宫崎F株的细胞色素c(3)在兼性需氧菌奥奈达希瓦氏菌MR-1中于厌氧、微需氧和好氧条件下均成功表达,产量为0.3至0.5毫克细胞色素/克细胞。含有来自普通脱硫弧菌宫崎F株细胞色素c(3)基因的广宿主范围质粒pRK415的衍生物用于转化奥奈达希瓦氏菌MR-1,产生的蛋白质产物与普通脱硫弧菌宫崎F株产生的蛋白质产物难以区分,只是在N端存在一个额外的丙氨酸残基。