牛传染性胸膜肺炎T1疫苗株的特异性PCR鉴定

Specific PCR identification of the T1 vaccine strains for contagious bovine pleuropneumonia.

作者信息

Lorenzon S, David A, Nadew M, Wesonga H, Thiaucourt F

机构信息

CIRAD-EMVT, FAO World Reference Laboratory for CBPP, BP 5035 Montpellier, Cedex 1, France.

出版信息

Mol Cell Probes. 2000 Aug;14(4):205-10. doi: 10.1006/mcpr.2000.0305.

DOI:10.1006/mcpr.2000.0305

PMID:10970724

Abstract

A specific PCR test for the identification of the vaccine strains T1, T1/44 and T1sr, was developed. This PCR reaction is based on variations of DNA sequences in a region flanking one IS1296 copy. The specific primer pair MmmSCP1-T1M2 amplifies a 700-bp long DNA fragment in the T1 vaccine strains and gives no amplification with the 60 other Mycoplasma mycoides subsp. mycoides SC strains tested. This PCR will permit to distinguish the T1 strain from all other vaccine strains and therefore avoid possible confusions. In addition, it should enable better investigations of post-vaccinal reactions.

摘要

开发了一种用于鉴定疫苗株T1、T1/44和T1sr的特异性PCR检测方法。该PCR反应基于一个IS1296拷贝侧翼区域DNA序列的变异。特异性引物对MmmSCP1-T1M2在T1疫苗株中扩增出一条700bp长的DNA片段，而在所测试的其他60株蕈状支原体丝状亚种SC菌株中无扩增。这种PCR将能够区分T1株与所有其他疫苗株，从而避免可能的混淆。此外，它应该能够更好地研究疫苗接种后的反应。