亚细胞组分的电子显微镜检查。I. 从颗粒悬浮液中制备代表性样品。
Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.
作者信息
Baudhuin P, Evrard P, Berthet J
出版信息
J Cell Biol. 1967 Jan;32(1):181-91. doi: 10.1083/jcb.32.1.181.
Abstract
A method is described for preparing, by filtration on Millipore filters, very thin (about 10 micro) pellicles of packed particles. These pellicles can be embedded in Epon for electron microscopic examination. They are also suitable for cytochemical assays. The method was used with various particulate fractions from rat liver. Its main advantages over the usual centrifugal packing techniques are that it produces heterogeneity solely in the direction perpendicular to the surface of the pellicle and that sections covering the whole depth of the pellicle can be photographed in a single field. It thus answers the essential criterion of random sampling and can be used for accurate quantitative evaluations.
摘要
本文描述了一种通过在微孔滤膜上过滤来制备紧密堆积颗粒的极薄（约10微米）薄膜的方法。这些薄膜可包埋在环氧树脂中用于电子显微镜检查。它们也适用于细胞化学分析。该方法用于大鼠肝脏的各种颗粒级分。与通常的离心堆积技术相比，其主要优点是它仅在垂直于薄膜表面的方向上产生异质性，并且可以在单个视野中拍摄覆盖薄膜整个深度的切片。因此，它符合随机抽样的基本标准，可用于准确的定量评估。
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