Zhang H, Yoshida S, Aizawa T, Murakami R, Suzuki M, Koganezawa N, Matsuura A, Miyazawa M, Kawano K, Nitta K, Kato Y
National Institute of Sericultural and Entomological Science, Tsukuba, Japan.
Antimicrob Agents Chemother. 2000 Oct;44(10):2701-5. doi: 10.1128/AAC.44.10.2701-2705.2000.
ASABF is a CSalphabeta-type antimicrobial peptide that contains four intramolecular disulfide bridges (Y. Kato and S. Komatsu, J. Biol. Chem. 271:30493-30498, 1996). In the present study, a recombinant ASABF was produced by using a yeast expression system, and its antimicrobial activity was characterized in detail. The recombinant ASABF was active against all gram-positive bacteria tested (7 of 7; minimum bactericidal concentration [MBC], 0.03 to 1 microg/ml) except Leuconostoc mesenteroides, some gram-negative bacteria (8 of 14; MBC, >0.5 microg/ml), and some yeasts (3 of 9; MBC >3 microg/ml). Slight hemolytic activity (4.2% at 100 microg/ml) against human erythrocytes was observed only under low-ionic-strength conditions. Less than 1 min of contact was enough to kill Staphylococcus aureus ATCC 6538P. The bactericidal activity against S. aureus was inhibited by salts.
ASABF是一种CSαβ型抗菌肽,含有四个分子内二硫键(Y. 加藤和S. 小松,《生物化学杂志》271:30493 - 30498,1996)。在本研究中,通过酵母表达系统产生了重组ASABF,并对其抗菌活性进行了详细表征。重组ASABF对除肠系膜明串珠菌外的所有测试革兰氏阳性菌(7株中的7株;最低杀菌浓度[MBC],0.03至1微克/毫升)、一些革兰氏阴性菌(14株中的8株;MBC,>0.5微克/毫升)和一些酵母(9株中的3株;MBC >3微克/毫升)具有活性。仅在低离子强度条件下观察到对人红细胞有轻微溶血活性(100微克/毫升时为4.2%)。接触不到1分钟就足以杀死金黄色葡萄球菌ATCC 6538P。对金黄色葡萄球菌的杀菌活性受到盐的抑制。