连接体介导的对N(10)-(3-磺丙基)-N-磺酰吖啶鎓-9-甲酰胺示踪剂化学发光信号的调节
Linker-mediated modulation of the chemiluminescent signal from N(10)-(3-Sulfopropyl)-N-sulfonylacridinium-9-carboxamide tracers.
作者信息
Adamczyk M, Chen Y Y, Fishpaugh J R, Mattingly P G, Pan Y, Shreder K, Yu Z
机构信息
Abbott Laboratories, Diagnostics Division, Department of Chemistry D9NM, Building AP 20, 100 Abbott Park Road, Abbott Park, Illinois 60064-6016, USA.
出版信息
Bioconjug Chem. 2000 Sep-Oct;11(5):714-24. doi: 10.1021/bc0000280.
Four chemiluminescent N-sulfonylacridinium-9-carboxamide active esters (17-20) were prepared from the corresponding acids and coupled to both of the aminated phenobarbital (13) and N-(6-aminohexyl)phenytoin (16) haptens. The level of signal produced by chemiluminescent N-sulfonylacridinium-9-carboxamide phenobarbital and phenytoin tracers in a solid-phase immunoassay format was found to be modulated by at least 20-fold by the judicious choice of the reactive acridinium-hapten linking group.
从相应的酸制备了四种化学发光的N-磺酰基吖啶-9-甲酰胺活性酯（17-20），并将其与胺化苯巴比妥（13）和N-（6-氨基己基）苯妥英（16）半抗原偶联。发现在固相免疫测定形式中，通过明智地选择反应性吖啶鎓-半抗原连接基团，化学发光的N-磺酰基吖啶-9-甲酰胺苯巴比妥和苯妥英示踪剂产生的信号水平至少可被调节20倍。
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