Sunshine M G, Sauer B
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2770-4. doi: 10.1073/pnas.72.7.2770.
A mutant of Escherichia coli strain C has been isolated, called gro109, that blocks bacteriophage P2 propagation by interfering with late gene expression. DNA replication proceeds normally in P2+-infected gro109 cells, but late phage proteins are not made. Early P2 mRNA is made in normal amounts, but very little late mRNA can be detected. P2 mutants (P2 ogr) able to overcome the gro109 block have been isolated in which synthesis of late P2 mRNA and phage proteins Is restored. The gro109 mutation is closely linked to the cluster of ribosomal genes at 64 min and is recessive to the wild-type (gro+) allele. A P2 ogr mutation has been mapped on the left arm of the P2 genome, between the right-most known late gene (D) and the phage attachment site. P2 ogr can complement P2+ in gro109 cells, indicating that ogr codes for a diffusible product.
已分离出大肠杆菌C菌株的一种突变体，称为gro109，它通过干扰晚期基因表达来阻断噬菌体P2的繁殖。在感染了P2 +的gro109细胞中，DNA复制正常进行，但晚期噬菌体蛋白无法合成。早期P2 mRNA的合成量正常，但几乎检测不到晚期mRNA。已分离出能够克服gro109阻断的P2突变体（P2 ogr），其中晚期P2 mRNA和噬菌体蛋白的合成得以恢复。gro109突变与位于64分钟处的核糖体基因簇紧密连锁，并且对野生型（gro +）等位基因呈隐性。一个P2 ogr突变已定位在P2基因组的左臂上，在最右侧已知的晚期基因（D）和噬菌体附着位点之间。P2 ogr可以在gro109细胞中互补P2 +，这表明ogr编码一种可扩散的产物。
