印度南部HIV血清阳性受试者中2型人类免疫缺陷病毒（HIV）的分子确认
Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in south India.
作者信息
Kannangai R, Ramalingam S, Prakash K J, Abraham O C, George R, Castillo R C, Schwartz D H, Jesudason M V, Sridharan G
机构信息
Departments of Clinical Virology, Christian Medical College Hospital, Vellore, India.
出版信息
Clin Diagn Lab Immunol. 2000 Nov;7(6):987-9. doi: 10.1128/CDLI.7.6.987-989.2000.
Nested PCRs for human immunodeficiency virus type 1 (HIV-1) and HIV-2 were compared with immunoblot test results. Twelve of 13 immunoblot-positive HIV-2 samples were positive by PCR. There were five INNO-LIA (Innogenetics, Zwijnaarde, Belgium) and/or HIVBLOT 2.2 (Genelabs, Singapore) samples that tested positive for dual infection. HIV-1 PCR was positive in all samples, while HIV-2 PCR was positive in two and RIBA (Chiron Corporation, San Diego, Calif.) was positive for HIV-2 in three samples. Thus the prevalence of HIV-2 is accurately estimated by the use of immunoblotting, but that of HIV-1 and -2 dual infection may be overestimated.
将用于1型人类免疫缺陷病毒（HIV-1）和HIV-2的巢式聚合酶链反应（Nested PCR）与免疫印迹检测结果进行了比较。13份免疫印迹阳性的HIV-2样本中有12份通过PCR检测呈阳性。有5份INNO-LIA（Innogenetics公司，比利时兹韦纳尔德）和/或HIVBLOT 2.2（Genelabs公司，新加坡）样本检测出双重感染呈阳性。所有样本的HIV-1 PCR均呈阳性，而HIV-2 PCR在两份样本中呈阳性，RIBA（Chiron公司，加利福尼亚州圣地亚哥）在三份样本中对HIV-2呈阳性。因此，通过免疫印迹法可准确估计HIV-2的流行率，但HIV-1和-2双重感染的流行率可能被高估。
Zotero 插件