高水平耐氟喹诺酮类的大肠杆菌临床分离株会过量产生多药外排蛋白AcrA。
High-level fluoroquinolone-resistant clinical isolates of Escherichia coli overproduce multidrug efflux protein AcrA.
作者信息
Mazzariol A, Tokue Y, Kanegawa T M, Cornaglia G, Nikaido H
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206, USA.
出版信息
Antimicrob Agents Chemother. 2000 Dec;44(12):3441-3. doi: 10.1128/AAC.44.12.3441-3443.2000.
Abstract
Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that this protein was overexpressed by >/=170% in 9 of 10 clinical isolates of Esherichia coli with high-level ciprofloxacin resistance (MICs, >/=32 microg/ml) but not in any of the 15 isolates for which the MIC was </=1 microg/ml.
摘要
用抗AcrA（AcrAB多药外排系统的一个必需组分）抗体进行免疫印迹分析表明，在10株对环丙沙星具有高水平耐药性（MICs≥32μg/ml）的大肠杆菌临床分离株中，有9株该蛋白的表达量上调≥170%，而在15株MIC≤1μg/ml的分离株中，该蛋白均未出现过表达。
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