采用高pH值阴离子交换色谱-脉冲安培检测法分离和测定糖醇及糖类。

Separation and determination of alditols and sugars by high-pH anion-exchange chromatography with pulsed amperometric detection.

作者信息

Andersen R, Sørensen A

机构信息

Institute of Food Research and Nutrition, Division of Nutrition, Danish Veterinary and Food Administration, Søborg.

出版信息

J Chromatogr A. 2000 Nov 3;897(1-2):195-204. doi: 10.1016/s0021-9673(00)00783-4.

DOI:10.1016/s0021-9673(00)00783-4

PMID:11128203

Abstract

Carbohydrates such as alditols (polyols or sugar alcohols), monosaccharides and disaccharides are separated as anions by anion-exchange chromatography with a sodium hydroxide eluent, MA1 CarboPac column and pulsed amperometric detection. We report a high-pH anion-exchange chromatographic-pulsed amperometric detection (HPAEC-PAD) method that determines all the polyols used as food additives in food products and the most commonly found mono- and disaccharides on a routine basis. The linearity, repeatability, internal reproducibility and accuracy are described. The applicability of the method has been demonstrated by the analysis of 46 relevant samples and by participation twice in the Food Analysis Performance Assessment Scheme (FAPAS) testing programme for food additives.

摘要

诸如糖醇（多元醇或糖醇）、单糖和双糖之类的碳水化合物，通过使用氢氧化钠洗脱液、MA1 CarboPac柱和脉冲安培检测的阴离子交换色谱法作为阴离子进行分离。我们报告了一种高pH值阴离子交换色谱 - 脉冲安培检测（HPAEC - PAD）方法，该方法可常规测定食品中用作食品添加剂的所有多元醇以及最常见的单糖和双糖。描述了该方法的线性、重复性、内部重现性和准确性。通过对46个相关样品的分析以及两次参与食品添加剂的食品分析性能评估计划（FAPAS）测试程序，证明了该方法的适用性。